# N and O isotope effects of nitrate reductases # PI: Julie Granger (UConn) # Version: 12 Aug 2015 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 8 07102013 methyl_viologen cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 8.0 194.6 23.4 0.0 18.6 0.0 8.1 99.1 40.9 0.4 34.8 0.1 8.2 50.2 60.4 0.3 53.7 0.2 8.3 11.1 100.8 nd 94.0 nd ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 9 07102013 methyl_viologen cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 9.0 812.6 23.9 0.0 18.5 0.0 9.1 655.9 32.1 0.1 26.1 0.0 9.2 373.4 49.2 0.2 41.9 0.1 9.3 59.8 100.3 0.0 89.0 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 20 06182014 methyl_viologen cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 20.0 879.3 26.7 0.0 21.8 0.1 20.1 747.9 31.7 0.1 26.5 0.2 20.1 572.7 39.6 0.0 34.0 0.2 20.1 397.5 50.5 0.1 44.5 0.2 20.1 237.6 64.1 0.5 57.8 0.4 20.1 96.4 75.7 0.7 69.9 0.6 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 24 07102014 methyl_viologen cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 24.0 896.8 26.2 0.2 20.9 0.1 24.1 894.6 27.7 0.3 22.4 0.1 24.2 860.7 29.6 0.3 24.3 0.2 24.3 721.6 32.3 0.0 27.3 0.0 24.4 636.2 35.8 0.0 30.5 0.0 24.5 563.9 39.3 0.1 34.3 0.0 24.6 523.4 41.7 0.0 37.3 0.0 24.7 286.9 57.3 1.4 51.3 0.5 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 25 07102014 methyl_viologen cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 25.0 893.5 26.2 0.5 21.1 0.3 25.1 832.2 27.6 0.0 22.8 0.1 25.2 815.8 29.3 0.0 24.4 0.1 25.3 730.4 32.2 0.1 27.3 0.3 25.4 646.1 34.7 0.0 29.9 0.1 25.5 622.0 37.5 0.0 33.0 0.0 25.6 572.7 40.4 0.0 35.9 0.0 25.7 443.5 45.6 0.1 40.2 0.1 25.8 387.6 49.8 0.2 44.5 0.2 25.9 321.9 55.0 0.2 49.3 0.1 26.1 283.6 58.8 0.1 53.3 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 27 01102104 methyl_viologen cell_suspension r._sphaeroides no aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 27.0 349.9 26.0 0.0 20.4 0.1 27.1 304.9 30.0 0.3 22.0 0.0 27.2 282.4 33.3 0.3 24.0 0.1 27.3 253.1 36.3 0.0 25.5 0.0 27.4 231.8 39.3 0.0 27.0 0.1 27.5 216.0 41.5 0.1 28.0 0.0 27.6 199.1 46.4 0.1 30.4 0.0 27.7 189.0 47.7 0.2 31.2 0.0 27.8 185.6 50.4 0.0 32.6 0.1 27.9 167.6 52.8 0.0 33.7 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 28 01102104 methyl_viologen cell_suspension r._sphaeroides no aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 28.0 344.3 25.7 0.0 20.0 0.2 28.1 322.6 29.8 0.2 22.4 0.1 28.2 268.8 32.9 0.0 24.1 0.1 28.3 241.5 35.5 0.2 25.5 0.1 28.4 215.0 38.3 0.1 26.7 0.1 28.5 161.3 42.6 0.1 28.9 0.0 28.6 147.5 45.1 0.0 29.6 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 29 11222014 methyl_viologen pure_extract A._thalania nd nd 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 29.0 401.8 26.3 0.5 19.7 0.2 29.1 351.6 29.6 0.0 23.2 0.5 29.2 304.1 33.4 0.6 27.1 0.4 29.3 255.7 37.5 0.3 30.9 0.1 29.4 216.3 40.6 2.0 34.4 0.9 29.5 194.0 45.3 0.7 38.5 0.3 29.6 170.5 48.7 0.0 41.9 0.1 29.7 148.0 52.8 0.1 45.2 0.2 29.8 129.3 55.0 0.2 48.3 0.4 29.9 122.3 56.4 0.1 50.1 0.0 29.1 112.5 58.2 1.0 51.8 0.6 29.11 106.1 60.1 0.5 53.7 0.9 29.12 95.3 63.0 0.7 56.5 0.4 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 30 11222014 methyl_viologen pure_extract P._angusta nd nd 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 30.0 878.6 25.3 1.7 19.8 0.8 30.1 794.3 28.5 0.3 22.6 0.2 30.2 748.1 31.0 0.1 24.7 0.1 30.3 711.0 30.9 2.2 26.3 0.3 30.4 669.4 34.3 0.1 27.8 0.0 30.5 614.3 35.6 0.0 29.6 0.1 30.6 580.5 37.8 0.9 31.9 0.4 30.7 540.0 40.6 0.0 34.1 0.2 30.8 480.4 42.0 0.2 35.9 0.2 30.9 470.3 44.9 0.4 38.1 0.1 30.10 406.1 47.8 0.7 41.4 0.5 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 31 11222014 methyl_viologen pure_extract P._angusta nd nd 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 31.0 355.5 31.1 0.1 24.1 0.1 31.1 321.8 32.8 0.6 25.9 0.7 31.2 286.9 35.6 0.6 29.1 0.2 31.3 262.1 39.5 0.9 31.9 0.1 31.4 225.0 44.5 0.2 36.1 0.2 31.5 201.4 44.1 0.0 36.4 0.2 31.6 196.9 47.1 0.1 39.2 0.3 31.7 181.1 49.6 0.0 41.3 0.1 31.8 166.5 51.4 0.2 43.4 0.3 31.9 140.6 53.6 0.9 45.5 0.0